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a, IHC of <t>RORB</t> <t>(green),</t> <t>BHLHE22</t> (red) and VGLUT2 (blue) in coronal sections of PD 7 mouse brains from control and Meis2 cKO mice. b, Quantification of fluorescence intensity across cortical bins (lateral Bin1 to medial Bin10; Bin1–2, AI; Bin3–4, SSp; Bin5–8, MOs/p; Bin9–10, mPFC). In Meis2 cKO mice, RORB-, BHLHE22- and VGLUT2-positive signals expand into MC areas. c,d, IHC of VGLUT2 in coronal sections of control and Meis2 cKO mice at PCD 16 and PD 0, spanning the rostro–caudal cortical axis. Scale bars, see images; statistical details, see Methods.
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a, IHC of <t>RORB</t> <t>(green),</t> <t>BHLHE22</t> (red) and VGLUT2 (blue) in coronal sections of PD 7 mouse brains from control and Meis2 cKO mice. b, Quantification of fluorescence intensity across cortical bins (lateral Bin1 to medial Bin10; Bin1–2, AI; Bin3–4, SSp; Bin5–8, MOs/p; Bin9–10, mPFC). In Meis2 cKO mice, RORB-, BHLHE22- and VGLUT2-positive signals expand into MC areas. c,d, IHC of VGLUT2 in coronal sections of control and Meis2 cKO mice at PCD 16 and PD 0, spanning the rostro–caudal cortical axis. Scale bars, see images; statistical details, see Methods.
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a, IHC of <t>RORB</t> <t>(green),</t> <t>BHLHE22</t> (red) and VGLUT2 (blue) in coronal sections of PD 7 mouse brains from control and Meis2 cKO mice. b, Quantification of fluorescence intensity across cortical bins (lateral Bin1 to medial Bin10; Bin1–2, AI; Bin3–4, SSp; Bin5–8, MOs/p; Bin9–10, mPFC). In Meis2 cKO mice, RORB-, BHLHE22- and VGLUT2-positive signals expand into MC areas. c,d, IHC of VGLUT2 in coronal sections of control and Meis2 cKO mice at PCD 16 and PD 0, spanning the rostro–caudal cortical axis. Scale bars, see images; statistical details, see Methods.
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a, IHC of <t>RORB</t> <t>(green),</t> <t>BHLHE22</t> (red) and VGLUT2 (blue) in coronal sections of PD 7 mouse brains from control and Meis2 cKO mice. b, Quantification of fluorescence intensity across cortical bins (lateral Bin1 to medial Bin10; Bin1–2, AI; Bin3–4, SSp; Bin5–8, MOs/p; Bin9–10, mPFC). In Meis2 cKO mice, RORB-, BHLHE22- and VGLUT2-positive signals expand into MC areas. c,d, IHC of VGLUT2 in coronal sections of control and Meis2 cKO mice at PCD 16 and PD 0, spanning the rostro–caudal cortical axis. Scale bars, see images; statistical details, see Methods.
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a, IHC of <t>RORB</t> <t>(green),</t> <t>BHLHE22</t> (red) and VGLUT2 (blue) in coronal sections of PD 7 mouse brains from control and Meis2 cKO mice. b, Quantification of fluorescence intensity across cortical bins (lateral Bin1 to medial Bin10; Bin1–2, AI; Bin3–4, SSp; Bin5–8, MOs/p; Bin9–10, mPFC). In Meis2 cKO mice, RORB-, BHLHE22- and VGLUT2-positive signals expand into MC areas. c,d, IHC of VGLUT2 in coronal sections of control and Meis2 cKO mice at PCD 16 and PD 0, spanning the rostro–caudal cortical axis. Scale bars, see images; statistical details, see Methods.
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a, IHC of <t>RORB</t> <t>(green),</t> <t>BHLHE22</t> (red) and VGLUT2 (blue) in coronal sections of PD 7 mouse brains from control and Meis2 cKO mice. b, Quantification of fluorescence intensity across cortical bins (lateral Bin1 to medial Bin10; Bin1–2, AI; Bin3–4, SSp; Bin5–8, MOs/p; Bin9–10, mPFC). In Meis2 cKO mice, RORB-, BHLHE22- and VGLUT2-positive signals expand into MC areas. c,d, IHC of VGLUT2 in coronal sections of control and Meis2 cKO mice at PCD 16 and PD 0, spanning the rostro–caudal cortical axis. Scale bars, see images; statistical details, see Methods.
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Image Search Results


a, IHC of RORB (green), BHLHE22 (red) and VGLUT2 (blue) in coronal sections of PD 7 mouse brains from control and Meis2 cKO mice. b, Quantification of fluorescence intensity across cortical bins (lateral Bin1 to medial Bin10; Bin1–2, AI; Bin3–4, SSp; Bin5–8, MOs/p; Bin9–10, mPFC). In Meis2 cKO mice, RORB-, BHLHE22- and VGLUT2-positive signals expand into MC areas. c,d, IHC of VGLUT2 in coronal sections of control and Meis2 cKO mice at PCD 16 and PD 0, spanning the rostro–caudal cortical axis. Scale bars, see images; statistical details, see Methods.

Journal: bioRxiv

Article Title: A Retinoic Acid Autoregulatory Loop Governing Prefrontal-Motor Arealization

doi: 10.64898/2025.12.23.696038

Figure Lengend Snippet: a, IHC of RORB (green), BHLHE22 (red) and VGLUT2 (blue) in coronal sections of PD 7 mouse brains from control and Meis2 cKO mice. b, Quantification of fluorescence intensity across cortical bins (lateral Bin1 to medial Bin10; Bin1–2, AI; Bin3–4, SSp; Bin5–8, MOs/p; Bin9–10, mPFC). In Meis2 cKO mice, RORB-, BHLHE22- and VGLUT2-positive signals expand into MC areas. c,d, IHC of VGLUT2 in coronal sections of control and Meis2 cKO mice at PCD 16 and PD 0, spanning the rostro–caudal cortical axis. Scale bars, see images; statistical details, see Methods.

Article Snippet: Primary antibodies included: MEIS2 (1:1000, Santa Cruz Biotechnology, sc-81986), BCL11B (1:1000, Sigma-Aldrich, MABE1045), SATB2 (1:1000, Abcam, ab92446), PLXNC1 (1:250, R&D Systems, AF5375), SEMA7A (1:250, R&D Systems, AF1835), RORB (1:500, Novus Biologicals, NBP2-45610), BHLHE22 (1:1000, Sigma-Aldrich, HPA064872), VGLUT2 (1:500, Synaptic Systems, 135404), L1CAM (1:500, Millipore Sigma, MAB5272), tdTomato (1:2000, Scigen, AB8181), ALDH1A3 (1:500, Proteintech, 25167-1-AP; 1:500, Abcam, ab308527), FOXP2 (1:1000, Sigma-Aldrich, MABE415), GFP (1:2000, Aves Labs, NC9510598), NTNG1 (1:100, R&D Systems, AF1166), CASPASE3 (1:500, Cell Signaling Technology, 9661S), IBA1 (1:500, Cell Signaling Technology, 79394SF), and NeuN (1:500, Invitrogen, 702022).

Techniques: Control, Fluorescence